Summary
Type: SPE
Application #: 303700
Catalog#: 730014 - SPE Cartridges, Chromabond, C18 ec, 6ml, PP, 500mg, 30/pk
Detection: 50 µL were injected into a LC system.
Conditions: Column conditioning: With 3 mL MeOH, 3 mL water and 3 mL HSA buffer (0.02 M; 1.88 g sodium hexane-1-sulfonic acid in water, 1 mL acetic acid, diluted with water to 500 mL, pH 3.3).
Sample aspiration: The sample was passed through the column.
Column washing: The column was rinsed twice with 3 mL of water.
Elution: 2 mL eluent mixture (0.02 M HSA buffer: acetonitrile, 9:11).
Evaporation: The eluates were concentrated in a vacuum concentrator to < 1 g; the mass was made up to 1 g with water.
Authors: Suhren G Knappstein K
Source: Analyst 1998 123 2797-2801